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Image Search Results
Journal: bioRxiv
Article Title: The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in HCT116 colon cancer cells
doi: 10.1101/2023.03.17.533075
Figure Lengend Snippet: A . Immunofluorescence experiments performed on HCT116 cells treated, or not, with oxaliplatin (0.5 μM) monitoring g-H2AX (yellow). DAPI (blue) was used to stain DNA and the nuclei. Quantification of the staining is shown on the right side and is represented as the corrected nuclear fluorescence. Data are represented as the mean ± SEM. (n=3). Unpaired two-tailed Student’s t-test; ** p<0.01. B . Immunofluorescence experiments performed on HCT116 cells treated, or not, with oxaliplatin (0.5 μM) monitoring 53BP1 (grey). DAPI (blue) was used to stain DNA and the nuclei. Quantification of the staining is shown on the right side and is represented as the corrected nuclear fluorescence. Data are represented as the mean ± SEM. (n=3). Unpaired two-tailed Student’s t-test; *** p<0.001. C . Western blot analysis showing protein expression of TEAD4 and p53 family of proteins in HCT116 treated (Oxa), or not (NT) with oxaliplatin (0.5 μM). GAPDH was used as a loading control (n=3). D. Heat map corresponding to the genes differentially expressed in HCT116 cells after 24h of oxaliplatin treatment at IC50 (see Supplemental Table 1). The three replicates for the non-treated (NT) and treated (Oxa) are shown.
Article Snippet: Antibodies used were rabbit anti-53BP1 (1/100;
Techniques: Immunofluorescence, Staining, Fluorescence, Two Tailed Test, Western Blot, Expressing